The RefSeq mRNAs gene track for the 05 Oct 2020 Naegleria fowleri/GCA_014843625.1_ASM1484362v1 genome assembly displays translated blat alignments of vertebrate and invertebrate mRNA in GenBank.
Download GCA_014843625.1_ASM1484362v1.xenoRefGene.gtf.gz GTF file.
Total genome size: 27,994,426
Gene count: 897
Bases in genes: 1,362,814
Percent genome coverage: % 4.868
The mRNAs were aligned against the Naegleria fowleri/GCA_014843625.1_ASM1484362v1 genome using translated blat. When a single mRNA aligned in multiple places, the alignment having the highest base identity was found. Only those alignments having a base identity level within 1% of the best and at least 25% base identity with the genomic sequence were kept.
Specifically, the translated blat command is:
blat -noHead -q=rnax -t=dnax -mask=lower target.fa query.fa target.query.psl where target.fa is one of the chromosome sequence of the genome assembly, and the query.fa is the mRNAs from RefSeqThe resulting PSL outputs are filtered:
pslCDnaFilter -minId=0.35 -minCover=0.25 -globalNearBest=0.0100 -minQSize=20
-ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap
all.results.psl GCA_014843625.1_ASM1484362v1.xenoRefGene.psl
The filtered GCA_014843625.1_ASM1484362v1.xenoRefGene.psl is converted to
genePred data to display for this track.
The mRNA track was produced at UCSC from mRNA sequence data submitted to the international public sequence databases by scientists worldwide.
Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW. GenBank. Nucleic Acids Res. 2013 Jan;41(Database issue):D36-42. PMID: 23193287; PMC: PMC3531190
Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. GenBank: update. Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6. PMID: 14681350; PMC: PMC308779
Kent WJ. BLAT - the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64. PMID: 11932250; PMC: PMC187518