The RefSeq mRNAs gene track for the 15 Aug 2014 Toxoplasma gondii MAS/GCA_000224865.2_TGMAS1_v2 genome assembly displays translated blat alignments of vertebrate and invertebrate mRNA in GenBank.
Download GCA_000224865.2_TGMAS1_v2.xenoRefGene.gtf.gz GTF file.
Total genome size: 63,320,459
Gene count: 475
Bases in genes: 1,683,605
Percent genome coverage: % 2.659
The mRNAs were aligned against the Toxoplasma gondii MAS/GCA_000224865.2_TGMAS1_v2 genome using translated blat. When a single mRNA aligned in multiple places, the alignment having the highest base identity was found. Only those alignments having a base identity level within 1% of the best and at least 25% base identity with the genomic sequence were kept.
Specifically, the translated blat command is:
blat -noHead -q=rnax -t=dnax -mask=lower target.fa query.fa target.query.psl where target.fa is one of the chromosome sequence of the genome assembly, and the query.fa is the mRNAs from RefSeqThe resulting PSL outputs are filtered:
pslCDnaFilter -minId=0.35 -minCover=0.25 -globalNearBest=0.0100 -minQSize=20
-ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap
all.results.psl GCA_000224865.2_TGMAS1_v2.xenoRefGene.psl
The filtered GCA_000224865.2_TGMAS1_v2.xenoRefGene.psl is converted to
genePred data to display for this track.
The mRNA track was produced at UCSC from mRNA sequence data submitted to the international public sequence databases by scientists worldwide.
Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW. GenBank. Nucleic Acids Res. 2013 Jan;41(Database issue):D36-42. PMID: 23193287; PMC: PMC3531190
Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. GenBank: update. Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6. PMID: 14681350; PMC: PMC308779
Kent WJ. BLAT - the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64. PMID: 11932250; PMC: PMC187518