The file: sacCer2.gc5Base.varStep.txt.gz is the raw data used to encode the gc5Base track on sacCer2. This file was produced from the sacCer2.2bit sequence with the kent source tree utility hgGcPercent thusly: hgGcPercent -wigOut -doGaps -file=stdout -win=5 sacCer2 sacCer2.2bit \ | gzip > sacCer2.gc5Base.varStep.txt.gz The format of the data is variableStep wiggle data described at: http://genome.ucsc.edu/goldenPath/help/wiggle.html with a "span" size of 5 bases. Each value is for a window size of 5 bases. Thus, possible values are: 0, 20, 40, 60, 80, 100 for a GC count of: 0, 1, 2, 3, 4, 5 respectively. Odd values can occur when fewer than 5 bases are used in the calculation depending upon the location of gaps in the sequence. Note: the chromosome coordinates given are 1-relative coordinates. The first nucleotide on a chromosome is numbered 1. Example data from chrI: variableStep chrom=chrI span=5 1 60 6 60 11 80 16 60 ... etc ... Meaning base positions 1 through 5 have value 60, base positions 6 through 10 have value 60, base positions 11 through 15 have value 80, etc ...