This directory contains alignments of the following assemblies: - target/reference: P. pacificus (priPac1, Feb. 2007, Washington University School of Medicine GSC Pristionchus pacificus 5.0) - query: C. elegans (ce4, Jan. 2007, Washington University School of Medicine GSC and Sanger Institute WS170) Files included in this directory: - md5sum.txt: md5sum checksums for the files in this directory - priPac1.ce4.all.chain.gz: chained blastz alignments. The chain format is described in http://genome.ucsc.edu/goldenPath/help/chain.html . - priPac1.ce4.net.gz: "net" file that describes rearrangements between the species and the best C. elegans match to any part of the P. pacificus genome. The net format is described in http://genome.ucsc.edu/goldenPath/help/net.html . - axtNet/*.priPac1.ce4.net.axt.gz: chained and netted alignments, i.e. the best chains in the P. pacificus genome, with gaps in the best chains filled in by next-best chains where possible. The axt format is described in http://genome.ucsc.edu/goldenPath/help/axt.html . The chainSwap program was used to translate ce4-referenced chained blastz alignments to priPac1 into priPac1-referenced chains aligned to ce4. See the download directory goldenPath/ce4/vsPriPac1/README.txt for more information about the ce4-referenced blastz and chaining process. Chained alignments were processed into nets by the chainNet, netSyntenic, and netClass programs. Best-chain alignments in axt format were extracted by the netToAxt program. All programs run after blastz were written by Jim Kent at UCSC. ---------------------------------------------------------------- If you plan to download a large file or multiple files from this directory, we recommend you use ftp rather than downloading the files via our website. To do so, ftp to hgdownload.cse.ucsc.edu, then go to the directory goldenPath/priPac1/vsCe4/. To download multiple files, use the "mget" command: mget ... - or - mget -a (to download all files in the current directory) All files in this directory are freely available for public use. -------------------------------------------------------------------- References Harris, R.S. (2007) Improved pairwise alignment of genomic DNA Ph.D. Thesis, The Pennsylvania State University Chiaromonte, F., Yap, V.B., and Miller, W. Scoring pairwise genomic sequence alignments. Pac Symp Biocomput 2002, 115-26 (2002). Kent, W.J., Baertsch, R., Hinrichs, A., Miller, W., and Haussler, D. Evolution's cauldron: Duplication, deletion, and rearrangement in the mouse and human genomes. Proc Natl Acad Sci USA 100(20), 11484-11489 (2003). Schwartz, S., Kent, W.J., Smit, A., Zhang, Z., Baertsch, R., Hardison, R., Haussler, D., and Miller, W. Human-mouse alignments with BLASTZ. Genome Res. 13(1), 103-7 (2003).