README ----------------------------------------------------------------------------------------------- * This directory consists of data files for one of the eight cell lines used in the RNA transcript map experiments for Phase 2 of Affymetrix/NCI Human Transcriptome project. * RNA samples were hybridized to 3 replicate arrays. * Data for the 'SK-N-AS' cell line is hosted here. * Ten Chromosomes (6, 7, 13, 14, 19, 20, 21, 22, X, Y) span 98 chips. * There are a total of 294 files in this folder (98x3=294) * The filenaming convention for these files is as follows: * cellLine_replicate_chipnum.gz * SK_1_27.gz => SK cell line, replicate #1, chip #27 * Phase2 chips were designed from DNA sequence of Human Genome version 30 of NCBI. (June 2002 release) * The chromosomes that appear on a given chip can be found in the file "phase2_chip_mapping.txt". This file is also located in this directory. * Each file has 14 tab seperated values. They are as follows: * pm probe sequence: 25mer sequence * sequence top strand: t/f (true/false) * sequence name: name of chromosome * sequence position: position of first base of the probe sequence on chromosome * pm x: x value of pm feature on chip * pm y: y value of pm feature on chip * mm x: x value of mm feature on chip * mm y: y value of mm feature on chip * pm intensity: intensity of pm feature * pm sd: standard deviation of pixel values in the pm feature * pm npixels: number of pixels in pm feature * mm intensity: intensity of mm feature * mm sd: standard deviation of pixel values in the mm feature * mm npixels: number of pixels in mm feature