This file is from: http://hgdownload.cse.ucsc.edu/goldenPath/micMur3/multiz3way/README.txt This directory contains compressed multiple alignments of the following assemblies to the Mouse lemur genome (micMur3, Feb. 2017): Assemblies used in these alignments: (alignment type) Mouse lemur - Microcebus murinus Feb. 2017 (Broad/Mmur-3.0) reference Bushbaby - Otolemur garnettii Mar. 2011 (Broad/otoGar3) syntenic Human - Homo sapiens Dec. 2013 (GRCh38/hg38) syntenic These alignments were prepared using the methods described in the track description file: http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=micMur3&g=cons3way based on the phylogenetic tree: micMur3.3way.nh. Files in this directory: - micMur3.3way.nh - phylogenetic tree used during the multiz multiple alignment - micMur3.3way.commonNames.nh - same as micMur3.3way.nh with the UCSC database names replaced by the common name for the species - micMur3.3way.scientificName.nh - same as micMur3.3way.nh with the UCSC database names replaced by the scientific name for the species - micMur3.3way.maf.gz - the multiple alignments on the Mouse lemur genome - upstream*.ensGene.maf.gz: alignments to regions (1000, 2000 and 5000) bases upstream of Ensembl genes. - md5sum.txt - md5 check sums of these files to verify correct download files The "alignments" directory contains compressed FASTA alignments for the CDS regions of the mouse lemur genome (micMur3, Feb. 2016) aligned to the assemblies. The micMur3.3way.maf.gz file contain all the alignments for the chromosomes in the Mouse lemur genome, including additional annotations to indicate gap context and genomic breaks for the sequence in the underlying genome assemblies. Note, the compressed data size of the maf file is 1.7 Gb, uncompressed is more than 6.2 Gb. For a description of multiple alignment format (MAF), see http://genome.ucsc.edu/goldenPath/help/maf.html. PhastCons conservation scores for these alignments are available at: http://hgdownload.cse.ucsc.edu/goldenPath/micMur3/phastCons3way PhyloP conservation scores for these alignments are available at: http://hgdownload.cse.ucsc.edu/goldenPath/micMur3/phyloP3way --------------------------------------------------------------- To download a large file or multiple files from this directory, we recommend that you use rsync or ftp rather than downloading the files via our website. There is approximately 240 Mb of compressed data in this directory. Via rsync: rsync -av --progress \ rsync://hgdownload.cse.ucsc.edu/goldenPath/micMur3/multiz3way/ ./ Via FTP: ftp hgdownload.cse.ucsc.edu user name: anonymous password: go to the directory goldenPath/micMur3/multiz3way To download multiple files from the UNIX command line, use the "mget" command. mget ... - or - mget -a (to download all the files in the directory) Use the "prompt" command to toggle the interactive mode if you do not want to be prompted for each file that you download. --------------------------------------------------------------- All the files in this directory are freely usable for any purpose. For data use restrictions regarding the individual genome assemblies, see http://genome.ucsc.edu/goldenPath/credits.html.