This directory contains Primer data for this ENCODE composite.

Primer data files include the sequences of the primers used in the experiments and
sequences for control sites in the ENCODE pilot ENr313 gene desert region on chr16.

Gene-targeted Primers: wgEncodeUw5cGeneTargetedPrimers.txt.tgz

Forward primers were designed to HindIII sites in a 230-415 kb sequence centered on the DNase I hypersensitive sites
of interest.  Reverse primers were designed to HindIII sites for all transcription starts extending
1 Mb on either side of the region targeted by the forward primer set.

Myc Primers: wgEncodeUw5cMycPrimers.txt.tgz

Forward primers were designed to HindIII fragments of 4.29 Mb section of human chromosome 8 centered on the
gene desert 5~R of the myc gene.  Reverse primers were designed to all HindIII fragments containing
refseq txStarts in a 7.6 Mb region extending > 2 Mb on either side of the forward primer set.