This directory contains the Drosophila pseudoobscura Freeze 1 assembly (dp2, Aug. 2003) from the Baylor College of Medicine HGSC Drosophila Genome Project. Files included in this directory: dp2.2bit - contains the complete D. pseudoobscura/dp2 genome sequence in the 2bit file format. While we are providing the .2bit for this assembly, we will continue to use the .nib files in the Genome Browser. Repeats from RepeatMasker and Tandem Repeats Finder (with period of 12 or less) are shown in lower case; non-repeating sequence is shown in upper case. The utility program, twoBitToFa (available from the kent src tree), can be used to extract .fa file(s) from this file. A pre-compiled version of the command line tool can be found at: http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/ See also: http://genome.ucsc.edu/admin/git.html http://genome.ucsc.edu/admin/jk-install.html est.fa.gz - D. pseudoobscura ESTs in GenBank. This sequence data is updated once a week via automatic GenBank updates. md5sum.txt - MD5 checksum of these files to verify correct transmission. mrna.fa .gz - D. pseudoobscura mRNA from GenBank. This sequence data is updated once a week via automatic GenBank updates. scaffoldFa.zip - The working draft sequence in one file per scaffold. Repeats from RepeatMasker and Tandem Repeats Finder (with period of 12 or less) are in lower case while non-repeating sequence is in upper case. The main assembly is in the chrN.fa files where N is the name of the scaffold. scaffoldFaMasked.zip - The working draft sequence in one file per scaffold. Repeats are masked by capital N's and non-repeating sequence is shown in upper case. scaffoldOut.zip - RepeatMasker .out file for scaffolds. These were created with RepeatMasker at the -s sensitive setting. scaffoldTrf.zip - Tandem Repeats Finder locations, filtered to keep repeats with period less than or equal to 12, translated into one .bed file per scaffold. xenoMrna.fa.gz - GenBank mRNAs from species other than that of the genome. This sequence data is updated once a week via automatic GenBank updates. --------------------------------------------------------------- If you plan to download a large file or multiple files from this directory, we recommend you use ftp rather than downloading the files via our website. To do so, ftp to hgdownload.cse.ucsc.edu, then go to the directory goldenPath/dp2/bigZips. To download multiple files, use the "mget" command: mget ... - or - mget -a (to download all the files in the directory) All the files in this directory are freely available for public use.