This directory contains reciprocal-best netted chains for dm2-droYak2. - dm2.droYak2.rbest.net.gz: dm2-referenced recip.best net to droYak2. - dm2ToDroYak2.rbest.chain.gz: chains extracted from the recip.best net -- these can be passed to the liftOver program to translate coords from dm2 to droYak2 through the recip.best net. - droYak2.dm2.rbest.net.gz: droYak2-referenced recip.best net. - droYak2ToDm2.rbest.chain.gz: recip.best "liftOver" chains. Here is how these files were generated, starting with the file dm2.droYak2.over.chain.gz which can be downloaded as http://hgwdev.cse.ucsc.edu/goldenPath/dm2/liftOver/dm2ToDroYak2.over.chain.gz and files which we call "chrom.sizes" locally but which can be downloaded as chromInfo.txt.gz in the database/ subdir on our main download server, hgdownload.cse.ucsc.edu. # Swap dm2-best chains to be droYak2-referenced: chainStitchId dm2.droYak2.over.chain.gz stdout \ | chainSwap stdin stdout \ | chainSort stdin droYak2.dm2.tBest.chain # Net those on droYak2 to get droYak2-ref'd reciprocal best net: chainPreNet droYak2.dm2.tBest.chain \ /cluster/data/{droYak2,dm2}/chrom.sizes stdout \ | chainNet -minSpace=1 stdin /cluster/data/{droYak2,dm2}/chrom.sizes \ stdout /dev/null \ | netSyntenic stdin droYak2.dm2.rbest.net # Extract droYak2-ref'd reciprocal best chain: netChainSubset droYak2.dm2.rbest.net droYak2.dm2.tBest.chain stdout \ | chainStitchId stdin droYak2.dm2.rbest.chain # Swap to get dm2-ref'd reciprocal best chain: chainSwap droYak2.dm2.rbest.chain stdout \ | chainSort stdin dm2.droYak2.rbest.chain # Net those on dm2 to get dm2-ref'd reciprocal best net: chainPreNet dm2.droYak2.rbest.chain \ /cluster/data/{dm2,droYak2}/chrom.sizes stdout \ | chainNet -minSpace=1 -minScore=0 \ stdin /cluster/data/{dm2,droYak2}/chrom.sizes stdout /dev/null \ | netSyntenic stdin dm2.droYak2.rbest.net # Compress and clean up. rm droYak2.dm2.tBest.chain gzip *.rbest.* md5sum *.rbest.*.gz > md5sum.txt