Assembly details This version was assembled from 2.05 million whole genome shotgun (WGS) reads, of which 88.2% were in read pairs. The sequence reads were generated at Washington University School of Medicine's Genome Sequencing Center and the Sanger Centre (Hinxton, UK). The Phusion (Mullikin, J) assembler was used to assemble the WGS reads into contigs based on overlap information, and then into supercontigs using read pair information to cross gaps. These supercontigs were assembled into mapped ultracontigs based on FPC fingerprint mapping (generated at Washington University School of Medicine's Genome Sequencing Center), with material from previously finished clones used to bridge gaps. Using the sequence data to choose primers along major ultracontigs, a genetic map (Ray Miller, Washington University School of Medicine) was constructed which yielded 6 chromosomes. The genetic mapping data along with data from 1:1 elegans:briggsae orthologs were used to refine ultracontigs and to order and orient the sequences along the C. briggsae chromosomes. Of the 108 megabase (Mb) genome, 89Mb of the sequence is ordered and oriented along the chromosomes. An additional 12Mb is localized to a specific chromosome but not ordered (and thus located in the _random bin) leaving only 7Mb currently unassigned. The N50 contig size in this assembly is 41 kb and the N50 supercontig size is 1450 kb. Most genes should be complete. The sequencing centers estimate that this whole genome shotgun assembly achieved 98% coverage of the genome. We continue to refine the genetic map and plan to release an updated genomic sequence. The cb2 sequence and annotation data may be downloaded from the UCSC Genome Browser FTP server or Downloads web page. The sequence data carry specific conditions for use. The cb2 annotation tracks were generated by UCSC and collaborators worldwide. See the Credits page for a detailed list of the organizations and individuals who contributed to the success of this release.