This directory contains the Oct. 2010 (WS225/caeAng1) assembly of the C. angaria genome (caeAng1, Division of Biology, California Institute of Technology(PS1010) ps10rel4 (GCA_000165025.1)), as well as repeat annotations and GenBank sequences. This assembly was produced by Division of Biology, California Institute of Technology For more information on the C. angaria genome, see the project website: http://www.ncbi.nlm.nih.gov/Traces/wgs/?val=AEHI01 This UCSC sequence was obtained from: ftp://ftp.ncbi.nlm.nih.gov/genbank/genomes/Eukaryotes/invertebrates/Caenorhabditis_sp_PS1010/ps1010rel4/ Files included in this directory: caeAng1.2bit - contains the complete C. angaria/caeAng1 genome sequence in the 2bit file format. Repeats from WindowMasker and Tandem Repeats Finder (with period of 12 or less) are shown in lower case; non-repeating sequence is shown in upper case. The utility program, twoBitToFa (available from the kent src tree), can be used to extract .fa file(s) from this file. A pre-compiled version of the command line tool can be found at: http://hgdownload.cse.ucsc.edu/admin/exe/linux.x86_64/ See also: http://genome.ucsc.edu/admin/git.html http://genome.ucsc.edu/admin/jk-install.html caeAng1.agp.gz - Description of how the assembly was generated from fragments. caeAng1.fa.gz - "Soft-masked" assembly sequence in one file. Repeats from WindowMasker and Tandem Repeats Finder (with period of 12 or less) are shown in lower case; non-repeating sequence is shown in upper case. caeAng1.fa.masked.gz - "Hard-masked" assembly sequence in one file. Repeats are masked by capital Ns; non-repeating sequence is shown in upper case. caeAng1.fa.out.gz - RepeatMasker .out file. RepeatMasker was run with the -s (sensitive) setting. *NOTE: the genome was masked with WindowMasker and Tandem Repeats Finder. RepeatMasker version: June 30 2010 (open-3-2-9) version of RepeatMasker CC RELEASE 20090604 chromWMSdust.bed.gz - bed file for the repeat areas identified by WindowMasker with the -sdust option. caeAng1.trf.bed.gz - Tandem Repeats Finder locations, filtered to keep repeats with period less than or equal to 12, and translated into UCSC's BED format. md5sum.txt - checksums of files in this directory caeAng1.chrom.sizes - Two-column tab-separated text file containing assembly sequence names and sizes. caeAng1.gc5Base.wigVarStep.gz - ascii data wiggle variable step values used - to construct the GC Percent track caeAng1.gc5Base.wig.gz - wiggle database table for the GC Percent track - this is an older standard alternative to the current - bigWig format of the track, sometimes usefull for analysis caeAng1.gc5Base.wib - binary data to correspond with the gc5Base.wig file see also: http://genome.ucsc.edu/goldenPath/help/wiggle.html and http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database for a discussion of how to use the wig.gz and .wib files for interaction with the GC percent data values caeAng1.chromAlias.txt - sequence name alias file, one line for each sequence name. First column is sequence name followed by tab separated alias names. ------------------------------------------------------------------ If you plan to download a large file or multiple files from this directory, we recommend that you use ftp rather than downloading the files via our website. To do so, ftp to hgdownload.cse.ucsc.edu [username: anonymous, password: your email address], then cd to the directory goldenPath/caeAng1/bigZips. To download multiple files, use the "mget" command: mget ... - or - mget -a (to download all the files in the directory) Alternate methods to ftp access. Using an rsync command to download the entire directory: rsync -avzP rsync://hgdownload.cse.ucsc.edu/goldenPath/caeAng1/bigZips/ . For a single file, e.g. chromFa.tar.gz rsync -avzP rsync://hgdownload.cse.ucsc.edu/goldenPath/caeAng1/bigZips/chromFa.tar.gz . Or with wget, all files: wget --timestamping 'ftp://hgdownload.cse.ucsc.edu/goldenPath/caeAng1/bigZips/*' With wget, a single file: wget --timestamping 'ftp://hgdownload.cse.ucsc.edu/goldenPath/caeAng1/bigZips/chromFa.tar.gz' -O chromFa.tar.gz To unpack the *.tar.gz files: tar xvzf .tar.gz To uncompress the fa.gz files: gunzip .fa.gz