This directory contains alignments of the following assemblies: - target/reference: Filarial worm (bruMal2, May. 2014 (WS244/B_malayi-3.1/bruMal2), University of Pittsburgh B. malayi genome project) - query: M. incognita (melInc2, Feb. 2008 (M. incognita WS245/PRJEA28837/melInc2), Sanger and Genoscope) Files included in this directory: - md5sum.txt: md5sum checksums for the files in this directory - bruMal2.melInc2.all.chain.gz: chained blastz alignments. The chain format is described in http://genome.ucsc.edu/goldenPath/help/chain.html . - bruMal2.melInc2.net.gz: "net" file that describes rearrangements between the species and the best M. incognita match to any part of the Filarial worm genome. The net format is described in http://genome.ucsc.edu/goldenPath/help/net.html . - bruMal2.melInc2.net.axt.gz: chained and netted alignments, i.e. the best chains in the Filarial worm genome, with gaps in the best chains filled in by next-best chains where possible. The axt format is described in http://genome.ucsc.edu/goldenPath/help/axt.html . - bruMal2.melInc2.synNet.maf.gz - filtered net file for syntenic alignments only, in MAF format, see also, description of MAF format: http://genome.ucsc.edu/FAQ/FAQformat.html#format5 - bruMal2.melInc2.syn.net.gz - filtered net file for syntenic alignments only - reciprocalBest/ directory, contains reciprocal-best netted chains for bruMal2-melInc2 The chainSwap program was used to translate melInc2-referenced chained blastz alignments to bruMal2 into bruMal2-referenced chains aligned to melInc2. See the download directory goldenPath/melInc2/vsBruMal2/README.txt for more information about the melInc2-referenced blastz and chaining process. Chained alignments were processed into nets by the chainNet, netSyntenic, and netClass programs. Best-chain alignments in axt format were extracted by the netToAxt program. All programs run after blastz were written by Jim Kent at UCSC. ---------------------------------------------------------------- If you plan to download a large file or multiple files from this directory, we recommend you use ftp rather than downloading the files via our website. To do so, ftp to hgdownload.cse.ucsc.edu, then go to the directory goldenPath/bruMal2/vsMelInc2/. To download multiple files, use the "mget" command: mget ... - or - mget -a (to download all files in the current directory) All files in this directory are freely available for public use. -------------------------------------------------------------------- References Harris, R.S. (2007) Improved pairwise alignment of genomic DNA Ph.D. Thesis, The Pennsylvania State University Chiaromonte F, Yap VB, Miller W. Scoring pairwise genomic sequence alignments. Pac Symp Biocomput. 2002:115-26. Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D. Evolution's cauldron: Duplication, deletion, and rearrangement in the mouse and human genomes. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11484-9. Schwartz S, Kent WJ, Smit A, Zhang Z, Baertsch R, Hardison RC, Haussler D, Miller W. Human-Mouse Alignments with BLASTZ. Genome Res. 2003 Jan;13(1):103-7.