This directory contains the Apr. 2018 (ARS-UCD1.2/bosTau9) assembly of the cow genome (bosTau9, USDA ARS), as well as repeat annotations and GenBank sequences. For more information about this assembly, please note the NCBI resources: https://www.ncbi.nlm.nih.gov/genome/82 https://www.ncbi.nlm.nih.gov/genome/assembly/1677391 https://www.ncbi.nlm.nih.gov/bioproject/391427 https://www.ncbi.nlm.nih.gov/bioproject/SAMN03145444 Files included in this directory: bosTau9.2bit - contains the complete cow/bosTau9 genome sequence in the 2bit file format. Repeats from RepeatMasker and Tandem Repeats Finder (with period of 12 or less) are shown in lower case; non-repeating sequence is shown in upper case. The utility program, twoBitToFa (available from the kent src tree), can be used to extract .fa file(s) from this file. A pre-compiled version of the command line tool can be found at: http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86_64/ See also: http://genome.ucsc.edu/admin/git.html http://genome.ucsc.edu/admin/jk-install.html bosTau9.chrom.sizes - Two-column tab-separated text file containing assembly sequence names and sizes. bosTau9.agp.gz - Description of how the assembly was generated from fragments. bosTau9.fa.gz - "Soft-masked" assembly sequence in one file. Repeats from RepeatMasker and Tandem Repeats Finder (with period of 12 or less) are shown in lower case; non-repeating sequence is shown in upper case. bosTau9.fa.masked.gz - "Hard-masked" assembly sequence in one file. Repeats are masked by capital Ns; non-repeating sequence is shown in upper case. bosTau9.fa.out.gz - RepeatMasker .out file. RepeatMasker was run with the -s (sensitive) setting. February 01 2017 (open-4-0-7) 1.331 version of RepeatMasker RepBase library: Dfam_Consensus RELEASE 20170127 CC RepBase RELEASE 20170127; * bosTau9.trf.bed.gz - Tandem Repeats Finder locations, filtered to keep repeats with period less than or equal to 12, and translated into UCSC's BED format. md5sum.txt - checksums of files in this directory mrna.fa.gz - Cow mRNA from GenBank. This sequence data is updated once a week via automatic GenBank updates. refMrna.fa.gz - RefSeq mRNA from the same species as the genome. This sequence data is updated once a week via automatic GenBank updates. upstream1000.fa.gz - Sequences 1000 bases upstream of annotated transcription starts of RefSeq genes with annotated 5' UTRs. This file is updated weekly so it might be slightly out of sync with the RefSeq data which is updated daily for most assemblies. upstream2000.fa.gz - Same as upstream1000, but 2000 bases. upstream5000.fa.gz - Same as upstream1000, but 5000 bases. bosTau9.gc5Base.wigVarStep.gz - ascii data wiggle variable step values used - to construct the GC Percent track bosTau9.gc5Base.wig.gz - wiggle database table for the GC Percent track - this is an older standard alternative to the current - bigWig format of the track, sometimes usefull for analysis bosTau9.gc5Base.wib - binary data to correspond with the gc5Base.wig file see also: http://genome.ucsc.edu/goldenPath/help/wiggle.html and http://genomewiki.ucsc.edu/index.php/Using_hgWiggle_without_a_database for a discussion of how to use the wig.gz and .wib files for interaction with the GC percent data values bosTau9.chromAlias.txt - sequence name alias file, one line for each sequence name. First column is sequence name followed by tab separated alias names. ------------------------------------------------------------------ If you plan to download a large file or multiple files from this directory, we recommend that you use ftp rather than downloading the files via our website. To do so, ftp to hgdownload.soe.ucsc.edu [username: anonymous, password: your email address], then cd to the directory goldenPath/bosTau9/bigZips. To download multiple files, use the "mget" command: mget ... - or - mget -a (to download all the files in the directory) Alternate methods to ftp access. Using an rsync command to download the entire directory: rsync -avzP rsync://hgdownload.soe.ucsc.edu/goldenPath/bosTau9/bigZips/ . For a single file, e.g. chromFa.tar.gz rsync -avzP rsync://hgdownload.soe.ucsc.edu/goldenPath/bosTau9/bigZips/chromFa.tar.gz . Or with wget, all files: wget --timestamping 'ftp://hgdownload.soe.ucsc.edu/goldenPath/bosTau9/bigZips/*' With wget, a single file: wget --timestamping 'ftp://hgdownload.soe.ucsc.edu/goldenPath/bosTau9/bigZips/chromFa.tar.gz' -O chromFa.tar.gz To unpack the *.tar.gz files: tar xvzf .tar.gz To uncompress the fa.gz files: gunzip .fa.gz ----------------------------------------------------------------------------- GenBank Data Usage The GenBank database is designed to provide and encourage access within the scientific community to the most up to date and comprehensive DNA sequence information. Therefore, NCBI places no restrictions on the use or distribution of the GenBank data. However, some submitters may claim patent, copyright, or other intellectual property rights in all or a portion of the data they have submitted. NCBI is not in a position to assess the validity of such claims, and therefore cannot provide comment or unrestricted permission concerning the use, copying, or distribution of the information contained in GenBank. -----------------------------------------------------------------------------