Human Gene TAF4B (ENST00000269142.10_7) from GENCODE V45lift37
Description: Homo sapiens TATA-box binding protein associated factor 4b (TAF4B), transcript variant 2, mRNA. (from RefSeq NM_005640) RefSeq Summary (NM_005640): TATA binding protein (TBP) and TBP-associated factors (TAFs) participate in the formation of the TFIID protein complex, which is involved in initiation of transcription of genes by RNA polymerase II. This gene encodes a cell type-specific TAF that may be responsible for mediating transcription by a subset of activators in B cells. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]. Gencode Transcript: ENST00000269142.10_7 Gencode Gene: ENSG00000141384.13_10 Transcript (Including UTRs) Position: hg19 chr18:23,806,409-23,971,649 Size: 165,241 Total Exon Count: 15 Strand: + Coding Region Position: hg19 chr18:23,806,898-23,969,976 Size: 163,079 Coding Exon Count: 15
ID:TAF4B_HUMAN DESCRIPTION: RecName: Full=Transcription initiation factor TFIID subunit 4B; AltName: Full=Transcription initiation factor TFIID 105 kDa subunit; Short=TAF(II)105; Short=TAFII-105; Short=TAFII105; FUNCTION: Cell type-specific subunit of the general transcription factor TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF4B is a transcriptional coactivator of the p65/RELA NF- kappa-B subunit. Involved in the activation of a subset of antiapoptotic genes including TNFAIP3. May be involved in regulating folliculogenesis. Through interaction with OCBA/POU2AF1, acts as a coactivator of B-cell-specific transcription. Plays a role in spermiogenesis and oogenesis. SUBUNIT: TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs) (PubMed:15601843). Heterodimerizes with TAF12/TFII20 via the C-terminal H2A-like histone-fold domain (PubMed:15601843). This heterodimer forms a histone-like octamer with the TAF6/TAFII70-TAF9/TAFII31 heterodimer (PubMed:15601843). Interacts with P65/RELA homodimers and P65/RELA-REL heterodimers (PubMed:10849440). Interaction with POU2AF1, via its C-terminal activation domain, is required for octamer-dependent transcription (PubMed:10828057). Interacts with ZNF628 (By similarity). SUBCELLULAR LOCATION: Nucleus toplasm te=Export into the cytoplasm is mediated by a CRM1-independent nuclear export pathway and not by phosphorylation. TISSUE SPECIFICITY: Preferentially expressed in ovarian granulosa cells (at protein level). Highly expressed in B-cells. PTM: Under stimulation by forskolin, Isoform 1 is phosphorylated by protein kinase A (PKA). DISEASE: Spermatogenic failure 13 (SPGF13) [MIM:615841]: A disorder resulting in the absence (azoospermia) or reduction (oligozoospermia) of sperm in the semen, leading to male infertility. Note=The disease is caused by variants affecting the gene represented in this entry. MISCELLANEOUS: [Isoform 2]: May be due to exon inclusion. Unphosphorylated form. SIMILARITY: Belongs to the TAF4 family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF05236 - Transcription initiation factor TFIID component TAF4 family PF07531 - NHR1 homology to TAF
ModBase Predicted Comparative 3D Structure on Q92750
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Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0003677 DNA binding GO:0003700 transcription factor activity, sequence-specific DNA binding GO:0008134 transcription factor binding GO:0046982 protein heterodimerization activity GO:0051059 NF-kappaB binding
Biological Process: GO:0006352 DNA-templated transcription, initiation GO:0006355 regulation of transcription, DNA-templated GO:0006366 transcription from RNA polymerase II promoter GO:0006367 transcription initiation from RNA polymerase II promoter GO:0007283 spermatogenesis GO:0030154 cell differentiation GO:0045944 positive regulation of transcription from RNA polymerase II promoter GO:0048477 oogenesis GO:1901796 regulation of signal transduction by p53 class mediator
AB234096 - Homo sapiens taf4b mRNA for TATA box binding protein (TBP)-associated factor 4B, complete cds. AY888048 - Homo sapiens TAFII105 mRNA, complete cds, alternatively spliced. AY888049 - Homo sapiens TAFII105-E9 mRNA, complete cds, alternatively spliced. BC169304 - Synthetic construct Homo sapiens clone IMAGE:9093406 TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B) gene, partial cds. AB527735 - Synthetic construct DNA, clone: pF1KB6285, Homo sapiens TAF4B gene for TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa, without stop codon, in Flexi system. Y09321 - H.sapiens TAFII105 mRNA, partial. AK302593 - Homo sapiens cDNA FLJ59074 complete cds, highly similar to Transcription initiation factor TFIID 105 kDa subunit. JD055878 - Sequence 36902 from Patent EP1572962. JD192234 - Sequence 173258 from Patent EP1572962. JD268208 - Sequence 249232 from Patent EP1572962. BC169305 - Synthetic construct Homo sapiens clone IMAGE:9093241 TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa (TAF4B) gene, partial cds. JD436078 - Sequence 417102 from Patent EP1572962. JD558571 - Sequence 539595 from Patent EP1572962. JD508562 - Sequence 489586 from Patent EP1572962. BC032791 - Homo sapiens TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa, mRNA (cDNA clone IMAGE:3456971), with apparent retained intron. JD349001 - Sequence 330025 from Patent EP1572962. BC016360 - Homo sapiens TAF4b RNA polymerase II, TATA box binding protein (TBP)-associated factor, 105kDa, mRNA (cDNA clone IMAGE:4099482), with apparent retained intron. JD350143 - Sequence 331167 from Patent EP1572962. JD090944 - Sequence 71968 from Patent EP1572962. JD078332 - Sequence 59356 from Patent EP1572962. JD262241 - Sequence 243265 from Patent EP1572962. JD047196 - Sequence 28220 from Patent EP1572962. JD095956 - Sequence 76980 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q92750 (Reactome details) participates in the following event(s):
R-HSA-109636 Recognition and Binding of Core Promoter Elements by TFIID R-HSA-73946 Abortive initiation R-HSA-75856 Abortive Initiation Before Second Transition R-HSA-75873 Addition of Nucleotides 5 through 9 on the growing Transcript R-HSA-167136 Addition of nucleotides 5 through 9 on the growing HIV-1 transcript R-HSA-167474 Abortive HIV-1 Initiation Before Second Transition R-HSA-167477 Abortive HIV-1 initiation after formation of the first phosphodiester bond R-HSA-75866 Nucleophillic Attack by 3'-hydroxyl Oxygen of nascent transcript on the Alpha Phosphate of NTP R-HSA-75861 NTP Binds Active Site of RNA Polymerase II R-HSA-75864 Newly Formed Phosphodiester Bond Stabilized and PPi Released R-HSA-109637 Binding of TFIIA and TFIIB to the pol II promoter:TFIID complex R-HSA-75862 Fall Back to Closed Pre-initiation Complex R-HSA-75949 RNA Polymerase II Promoter Opening: First Transition R-HSA-167134 Newly formed phosphodiester bond stabilized and PPi released R-HSA-167130 Nucleophillic attack by 3'-hydroxyl oxygen of nascent HIV-1 transcript on the Alpha phosphate of NTP R-HSA-167118 NTP binds active site of RNA Polymerase II in HIV-1 open pre-initiation complex R-HSA-167484 Fall Back to Closed Pre-initiation Complex R-HSA-167097 HIV Promoter Opening: First Transition R-HSA-6805399 TAF1 phosphorylates TP53 R-HSA-109638 Recruitment of RNA Polymerase II Holoenzyme by TFIIF to the pol II promoter:TFIID:TFIIA:TFIIB complex R-HSA-75095 Binding of TFIIE to the growing preinitiation complex R-HSA-109639 Formation of the closed pre-initiation complex R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-73776 RNA Polymerase II Promoter Escape R-HSA-167162 RNA Polymerase II HIV Promoter Escape R-HSA-167161 HIV Transcription Initiation R-HSA-75953 RNA Polymerase II Transcription Initiation R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-167172 Transcription of the HIV genome R-HSA-73857 RNA Polymerase II Transcription R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation R-HSA-162599 Late Phase of HIV Life Cycle R-HSA-74160 Gene expression (Transcription) R-HSA-5633007 Regulation of TP53 Activity R-HSA-162587 HIV Life Cycle R-HSA-3700989 Transcriptional Regulation by TP53 R-HSA-162906 HIV Infection R-HSA-212436 Generic Transcription Pathway R-HSA-5663205 Infectious disease R-HSA-1643685 Disease
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
